Protective Effect of Edaravone in Primary Cerebellar Granule Neurons against Iodoacetic Acid-Induced Cell Injury

Published on Oct 18, 2015in Oxidative Medicine and Cellular Longevity5.076
· DOI :10.1155/2015/606981
Xinhua Zhou2
Estimated H-index: 2
(JNU: Jinan University),
Longjun Zhu3
Estimated H-index: 3
(JNU: Jinan University)
+ 7 AuthorsYuqiang Wang25
Estimated H-index: 25
(JNU: Jinan University)
Edaravone (EDA) is clinically used for treatment of acute ischemic stroke in Japan and China due to its potent free radical-scavenging effect. However, it has yet to be determined whether EDA can attenuate iodoacetic acid- (IAA-) induced neuronal death in vitro. In the present study, we investigated the effect of EDA on damage of IAA-induced primary cerebellar granule neurons (CGNs) and its possible underlying mechanisms. We found that EDA attenuated IAA-induced cell injury in CGNs. Moreover, EDA significantly reduced intracellular reactive oxidative stress production, loss of mitochondrial membrane potential, and caspase 3 activity induced by IAA. Taken together, EDA protected CGNs against IAA-induced neuronal damage, which may be attributed to its antiapoptotic and antioxidative activities.
📖 Papers frequently viewed together
21 Citations
26 Citations
#1Qing Ai (CQMU: Chongqing Medical University)H-Index: 11
#2Yuping Jing (CQMU: Chongqing Medical University)H-Index: 7
Last. Li Zhang (CQMU: Chongqing Medical University)H-Index: 24
view all 10 authors...
Abstract The syntheses of inflammatory mediators are energy-intensive processes and the mitochondria play pivotal roles in supporting these energy-requiring molecular responses. In the present studies, a mitochondrial respiratory complex I inhibitor rotenone was administrated in mice with lipopolysaccharide/D-galactosamine (LPS/D-Gal)-induced fulminant liver injury and the prophylactic and therapeutic effects on tissue injury were evaluated. We found that pretreatment with rotenone suppressed th...
17 CitationsSource
#1Theodore J. Kalogeris (MU: University of Missouri)H-Index: 24
#1Theodore J. Kalogeris (MU: University of Missouri)H-Index: 8
Last. Ronald J. Korthuis (MU: University of Missouri)H-Index: 9
view all 3 authors...
Reductions in the blood supply produce considerable injury if the duration of ischemia is prolonged. Paradoxically, restoration of perfusion to ischemic organs can exacerbate tissue damage and extend the size of an evolving infarct. Being highly metabolic organs, the heart and brain are particularly vulnerable to the deleterious effects of ischemia/reperfusion (I/R). While the pathogenetic mechanisms contributing to I/R-induced tissue injury and infarction are multifactorial, the relative import...
369 CitationsSource
#1Satoshi Ikeda (Kadai: Kagoshima University)H-Index: 8
#2Katsuhiro HaradaH-Index: 5
Last. Yurie KamikawaH-Index: 5
view all 4 authors...
Edaravone is a free radical scavenger that protects the adjacent cortex during cerebral infarction. We created a hemiparetic model of cerebral thrombosis from a photochemically induced infarction with the photosensitive dye, rose bengal, in rats. We examined the effects of edaravone on recovery in the model. A total of 36 adult Wistar rats were used. The right sensorimotor area was irradiated with green light with a wavelength of 533 nm (10 mm diameter), and the rose bengal was injected intraven...
6 CitationsSource
#1Guilian Zhang (Xi'an Jiaotong University)H-Index: 7
#2Wang-gang Zhang (Xi'an Jiaotong University)H-Index: 3
Last. Yingying Guo (Xi'an Jiaotong University)H-Index: 4
view all 12 authors...
Oxidative damage is an important mediator of Alzheimer’s disease (AD); hence, antioxidant therapy is a potential treatment for AD. Edaravone, a free radical scavenger, has been shown to have neuroprotective properties. The study aimed to examine the effects of edaravone on indicators of Aβ25-35-induced oxidative damage in PC12 cells. PC12 cells were treated with 20, 40, or 80 μM edaravone before treatment with 30 μM Aβ25-35. After treatment, the following assessments were performed: cell viabili...
21 CitationsSource
#1Qiqiang Tang (Anhui Medical University)H-Index: 3
#2Ruodong Han (Anhui Medical University)H-Index: 3
Last. Jun Li (Anhui Medical University)H-Index: 1
view all 6 authors...
Abstract The present study compared the potential neuroprotective effects of tanshinone (Tan) IIA monotherapy, tetramethylpyrazine (TMP) monotherapy, and Tan IIA+TMP combination therapy in adult rat subjected to cerebral ischemic injury using the permanent middle cerebral artery occlusion (MCAO) model and in primary cortical neuron culture exposed to oxygen-glucose deprivation (OGD) model. Male Sprague Dawley rats ( n =84) were randomly divided into sham-operated, MCAO, cmc-Na (sodium carboxymet...
36 CitationsSource
Results: mean mrS and nIHSS scores at 3 months were lowest in group e (p=0.000), suggestive of better outcome in this group. After application of severity scale, 15 patients (68.1%) in group-E, 14 (58.3%) in group C and 18 (72%) in group N had moderate to severe stroke (NIHSS of more than 10). When the patients of moderate to severe stroke were analyzed separately at 3 months, patients in group E (mean 4.46±3.52) had significantly (p=0.00) better outcome in comparison to group C (mean 10.28±7.93...
15 Citations
#1Marta Di CarloH-Index: 25
#2Daniela GiacomazzaH-Index: 21
Last. Pier Luigi San BiagioH-Index: 28
view all 5 authors...
Oxidative stress has long been linked to neuronal cell death that is associated with certain neurodegenerative conditions. Whether it is a primary cause or merely a downstream consequence of the neurodegenerative and aging process is still an open question. Mitochondria are deeply involved in the production of reactive oxygen species through the electron carriers of the respiratory chain and their role in neurodegenerative diseases is discussed here. Moreover, the input of new technological appr...
75 CitationsSource
#1Laura María Reyes-Fermín (UNAM: National Autonomous University of Mexico)H-Index: 7
#2Susana González-Reyes (UNAM: National Autonomous University of Mexico)H-Index: 10
Last. José Pedraza-Chaverri (UNAM: National Autonomous University of Mexico)H-Index: 73
view all 6 authors...
AbstractCurcumin is a phenolic yellow curry pigment with anti-inflammatory and antioxidant activities and α-mangostin is a xanthone isolated from mangosteen fruit with antioxidant properties. Iodoacetate (IAA) is an inhibitor of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase that induces a model of metabolic inhibition in neurons where reactive oxygen species (ROS) production is a significant mechanism. Furthermore, it has been shown that the induction of heme oxygenase-1 (HO-1) ...
45 CitationsSource
A major challenge in developing stroke therapeutics that augment adaptive pathways to stress has been to identify targets that can activate compensatory programs without inducing or adding to the stress of injury. In this regard, hypoxia-inducible factor prolyl hydroxylases (HIF PHDs) are central gatekeepers of posttranscriptional and transcriptional adaptation to hypoxia, oxidative stress, and excitotoxicity. Indeed, some of the known salutary effects of putative ‘antioxidant' iron chelators in...
78 CitationsSource
#1Jennifer M. Noel (University of Louisville)H-Index: 6
#2Juan P. Fernandez de Castro (University of Louisville)H-Index: 9
Last. Maureen A. McCall (University of Louisville)H-Index: 36
view all 11 authors...
Abstract Our purpose was to find a method to create a large animal model of inducible photoreceptor damage. To this end, we tested in domestic swine the efficacy of two chemical toxins, known to create photoreceptor damage in other species: Iodoacetic Acid (IAA) and Sodium Iodate (NaIO 3 ). Intravenous (IV) administration of NaIO 3 up to 90 mg/kg had no effect on retinal function and 110 mg/kg was lethal. IV administration of IAA (5–20 mg/kg) produced concentration-dependent changes in visual fu...
23 CitationsSource
Cited By10
#1Mengxiang Yang (NBU: Ningbo University)H-Index: 2
#2Yi Feng (NBU: Ningbo University)H-Index: 1
Last. Wei Cui (NBU: Ningbo University)H-Index: 21
view all 9 authors...
The main pathological features of ischemic stroke include neuronal damage and blood-brain barrier (BBB) dysfunction. Previous studies have shown that Evans Blue, a dye used to probe BBB integrity, could enter the brain only during the pathological status of ischemic stroke, indicating the potential pathologically activated therapeutic use of this chemical to treat ischemic stroke. In this study, we have reported that Evans Blue could produce in vitro neuroprotective effects against iodoacetic ac...
1 CitationsSource
#1Xiaofei Jiao (UIUC: University of Illinois at Urbana–Champaign)H-Index: 1
#2Andressa Gonsioroski (UIUC: University of Illinois at Urbana–Champaign)H-Index: 3
Last. Huanyu Qiao (UIUC: University of Illinois at Urbana–Champaign)H-Index: 8
view all 4 authors...
Disinfection by-products (DBPs) are compounds produced during the water disinfection process. Iodoacetic acid (IAA) is one of the unregulated DBPs in drinking water, with potent cytotoxicity and genotoxicity in animals. However, whether IAA has toxic effects on oocyte maturation remains unclear. Here, we show that IAA exposure resulted in metaphase I (MI) arrest and polar-body-extrusion failure in mouse oocytes, indicating that IAA had adverse effects on mouse oocyte maturation in vitro. Particu...
#1Gwang Myeong Noh (Kosin University)H-Index: 2
#2Jae Wan LimH-Index: 2
Last. Sang Joon Lee (Kosin University)H-Index: 11
view all 5 authors...
PURPOSE: We investigated structural changes in the retina by using optical coherence tomography (OCT) in a feline model of retinal degeneration using iodoacetic acid (IAA). METHODS: We examined 22 eyes of 11 felines over 2 years of age. The felines had fasted for 12 hours and were intravenously injected with IAA 20 mg/kg of body weight. OCT (Spectralis OCT) was performed at the point where the ends of the retinal vessels collected in the lateral direction from the optic nerve head and area centr...
#1Changzoo Kim (Kosin University)
#3Seung Uk Lee (Kosin University)H-Index: 6
Last. Sang Joon Lee (Kosin University)H-Index: 11
view all 4 authors...
#1Jing Wang (Yantai University)H-Index: 14
#2Rui Jia (Yantai University)H-Index: 3
Last. Wansong Zong (SDNU: Shandong Normal University)H-Index: 15
view all 5 authors...
Abstract Given the ubiquity of iodinated disinfection by-products (I-DBPs) in drinking water and their prominent toxicity, it is of vital significance to evaluate I-DBPs toxicity and explore the underlying mechanism. The toxicity of iodoacetic acid (IAA), a typical type of I-DBPs, might be linked with oxidative stress. However, it remains unknown for the response of antioxidant enzyme superoxide dismutase (SOD) in the mouse primary hepatocytes when exposed to IAA and the underlying mechanism. Th...
5 CitationsSource
#1Jing Wang (Yantai University)H-Index: 14
#2Rui Jia (Yantai University)H-Index: 3
Last. Wansong Zong (SDNU: Shandong Normal University)H-Index: 15
view all 6 authors...
Abstract Disinfection byproducts (DBPs) are produced during the disinfection of drinking water and pose a hazard to human health. As a typical type of DBPs, iodoacetic acid (IAA) exhibits prominent cytotoxicity in mammalian cell systems which links with oxidative stress. However, little is known about the relationship of catalase (CAT) with the cytotoxicity of IAA and the adverse effects of IAA to CAT. This study investigated the effects of IAA on the cell viability and CAT activity in the mouse...
8 CitationsSource
#1Gaoxiao Zhang (JNU: Jinan University)H-Index: 14
#2Tao Zhang (JNU: Jinan University)H-Index: 4
Last. Zaijun Zhang (JNU: Jinan University)H-Index: 22
view all 14 authors...
Our previous studies demonstrated that the multifunctional agent TBN, a derivative of tetramethylpyrazine armed with a nitrone moiety, displayed high therapeutic efficacy in experimental ischemic stroke models. However, its molecular mechanisms of action underlying the neuroprotective effect need further exploration. In the present study, we found that TBN had significant activities scavenging free radicals such as ·OH, O 2 ·− and ONOO−, inhibiting Ca2+ overload, maintaining mitochondrial functi...
7 CitationsSource
#1Hui Lin (HUST: Huazhong University of Science and Technology)H-Index: 8
#2Xuan Ma (HUST: Huazhong University of Science and Technology)H-Index: 3
Last. Zengwu Shao (HUST: Huazhong University of Science and Technology)H-Index: 27
view all 9 authors...
Abstract Aims Edaravone is a strong free radical scavenger most used for treating acute ischemic stroke. In this study we investigated the protective effects and underlying mechanisms of edaravone on compression-induced damage in rat nucleus pulposus (NP) cells. Materials and methods Cell viability was determined using MTT assay methods. NP cell apoptosis was measured by Hoechst 33,258 staining and Annexin V/PI double staining. Intracellular reactive oxygen species (ROS), mitochondrial membrane ...
8 CitationsSource
#1Zaijun Zhang (JNU: Jinan University)H-Index: 5
#2Gaoxiao ZhangH-Index: 14
Last. Yuqiang WangH-Index: 25
view all 13 authors...
Tetramethylpyrazine nitrone, a multifunctional neuroprotective agent for ischemic stroke therapy
17 CitationsSource