Maculas Affected by Age-Related Macular Degeneration Contain Increased Chelatable Iron in the Retinal Pigment Epithelium and Bruch's Membrane

Objective

To investigate whether iron is involved in the pathogenesis of age-related macular degeneration (AMD).

Methods

Postmortem AMD-affected (nonexudative or exudative) and healthy maculas were studied using the 3, 3′-diaminobenzidine–enhanced Perls Prussian blue stain. The Perls Prussian blue stain was quantified by computer-assisted analysis of digital images. To determine whether the iron was chelatable, sections treated...