Simultaneous determination of six isoflavonoids in rat plasma after administration of total flavonoid from Gegen by ultra-HPLC-MS/MS

A simple, specific, and sensitive ultra‐performance liquid chromatography‐tandem mass spectrometry method was developed for the simultaneous determination of 3′‐hydroxypuerarin, 6′′‐ O ‐xylosylpuerarin, mirificin, puerarin, 3′‐methoxypuerarin and daidzin in rat plasma. After the addition of methanol containing 0.1% formic acid and 10% ascorbic acid, the analytes and rutoside were obtained by protein precipitation, then separated on a Thermo...