Fragmentation of a highly purified monoclonal antibody attributed to residual CHO cell protease activity
Abstract
Monoclonal antibody (mAb) fragmentation can be a widespread problem across the biotechnology industry and there is a current need to better understand the underlying principles. Here, we report an example of a high-purity human IgG1 mAb prepared from CHO cells exhibiting fragmentation that can be attributed to residual proteolytic enzyme activity. The concomitant occurrence of proteolytic and non-proteolytic peptide bond cleavage is shown and...
Paper Details
Title
Fragmentation of a highly purified monoclonal antibody attributed to residual CHO cell protease activity
Published Date
Nov 17, 2010
Volume
108
Issue
4
Pages
977 - 982
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