A novel method for evaluating free radical scavenging abilities of antioxidants using ultraviolet induction of bacteriophage λ

A novel biological method used to evaluate free radical scavenging abilities of antioxidants using ultraviolet (UV) induction of bacteriophage λ in lysogenic Escherichia coli κ12 (λ+) has been developed. This method is based on the induction of bacteriophage λ from lysogenic cycle to lytic cycle by ultraviolet irradiation, and formation of free radicals during the course of induction. In the experiments, 108 cells/ml and 30 s (39 J/m2) were...