A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

Background Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody fragments cannot be used as intrabodies because they do not fold under the reducing conditions of the cell cytosol and nucleus. Results We describe the construction and validation of a large synthetic human single chain antibody...