Measurement of radical-scavenging ability in hepatic metallothionein of rat using in vivo electron spin resonance spectroscopy

In this study, the ability of metallothionein (MT) to scavenge free radicals was determined by in vivo electron spin resonance (ESR) spectroscopy using a carbamoyl-PROXYL, nitroxyl radical, as a spin probe. Production of metallothionein was induced in the liver of rats with ZnSO4 (0.2 mol/kg, ip) and the intensity of the carbamoyl-PROXYL ESR signal was measured at the upper abdominal level which is a position of the liver. After the injection of...