Improving mRNA 5′ coding sequence determination in the mouse genome

The incomplete determination of the mRNA 5′ end sequence may lead to the incorrect assignment of the first AUG codon and to errors in the prediction of the encoded protein product. Due to the significance of the mouse as a model organism in biomedical research, we performed a systematic identification of coding regions at the 5′ end of all known mouse mRNAs, using an automated expressed sequence tag (EST)-based approach which we have previously...