Evaluation of denaturing high‐performance liquid chromatography as a rapid detection method for identification of epidermal growth factor receptor mutations in nonsmall‐cell lung cancer

BACKGROUND. Somatic mutations of the epidermal growth factor receptor ( EGFR ) gene in nonsmall‐cell lung cancer (NSCLC) may predict responsiveness to tyrosine kinase inhibitors. These mutations are commonly identified using DNA sequencing methods. Although considered the gold standard, this approach is time‐consuming. In addition, this approach requires large diagnostic specimens and a high ratio of tumor‐to‐normal‐tissue DNA for optimal...