Surface-Initiated Enzymatic Polymerization of DNA
Abstract
We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3'-end of oligonucleotides. The thickness of the synthesized DNA layer was found to depend on the deoxymononucleotide monomer, in the order of dATP > dTTP > dGTP approximately dCTP. In addition, the composition and...
Paper Details
Title
Surface-Initiated Enzymatic Polymerization of DNA
Published Date
Oct 12, 2007
Journal
Volume
23
Issue
23
Pages
11712 - 11717
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