Transepidermal water loss reflects permeability barrier status: validation in human and rodent in vivo and ex vivo models

: Permeability barrier function is measured with instruments that assess transepidermal water loss (TEWL), either with closed‐ or open‐loop systems. Yet, the validity of TEWL as a measure of barrier status has been questioned recently. Hence, we tested the validity of this measure by comparing TEWL across a wide range of perturbations, with a variety of methods, and in a variety of models. TEWL rates with two closed‐chamber systems (VapoMeter...