Effect of organic loading rate on hydrogen production from sugarcane vinasse in thermophilic acidogenic packed bed reactors

Published on Oct 13, 2014in International Journal of Hydrogen Energy4.939
· DOI :10.1016/J.IJHYDENE.2014.08.017
Antônio Djalma Nunes Ferraz Júnior8
Estimated H-index: 8
(USP: University of São Paulo),
Jorge Wenzel8
Estimated H-index: 8
+ 1 AuthorsMarcelo Zaiat52
Estimated H-index: 52
(USP: University of São Paulo)
Sources
Abstract
Abstract This study evaluated the effect of organic loading rate (OLR) on hydrogen production in up-flow anaerobic packed bed reactors (APBR) continuously fed with sugarcane vinasse. Four thermophilic up-flow APBR were operated in parallel at different ORL. Continuous hydrogen production was detected. The optimum OLR of 84.2 kg-COD m−3 d−1 was assessed by polynomial adjustment, which predicted a maximum Volumetric Hydrogen Production (VHP) and hydrogen yield ( Y H 2 ) of 1117.2 mL-H2 d−1 L−1reactor and 2.4 mol-H2 mol−1total carbohydrates, respectively. The microbial composition was monitored using 16S rRNA gene by Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and quantification of Fe-hydrogenase gene by real-time PCR which was affected by the OLR. The number of the Fe-hydrogenase genes was proportional to the monitored hydrogen production and yield. Hydrogen-producing strains were isolated, and the 16S rRNA gene sequences were highly homologous to those of Thermoanaerobacterium thermosaccarolyticum. The ability of vinasse as substrate for hydrogen production was confirmed for both strains.
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#1Antônio Djalma Nunes Ferraz Júnior (USP: University of São Paulo)H-Index: 8
#2Marcelo Zaiat (USP: University of São Paulo)H-Index: 52
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Abstract This study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (UAnPBR). Two mesophilic UAPBRs (UAnPBR1 and 2) were tested at organic loading rates (OLRs) ranging from 6.5 to 51.4 gCOD L −1 d −1 . To overcome biomass washout, design modifications were made in the UAnPBR2 to include a settling zone to capture the detached biomass. The design modifications in UAnPBR2 increased the average hydrogen yield from 0.98 to...
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