An improved system for the generation and analysis of mutant proteins containing unnatural amino acids in Saccharomyces cerevisiae.

Volume: 371, Issue: 1, Pages: 112 - 122
Published: Aug 3, 2007
Abstract
We have previously described methodology that makes it possible to genetically encode a wide array of unnatural amino acids in both prokaryotic and eukaryotic organisms. Here, we report the systematic optimization of a Saccharomyces cerevisiae expression system for the production of mutant proteins containing unnatural amino acids. Modifications include significant increases in both the expression levels of the orthogonal Escherichia coli amber...
Paper Details
Title
An improved system for the generation and analysis of mutant proteins containing unnatural amino acids in Saccharomyces cerevisiae.
Published Date
Aug 3, 2007
Volume
371
Issue
1
Pages
112 - 122
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