Non‐invasive multiphoton imaging of extracellular matrix structures

Multiphoton microscopy has become a powerful method for the artifact‐free, nondestructive evaluation of deep‐tissue cells and extracellular matrix (ECM) structures in their native environment. By interacting with highly non‐centrosymmetric molecular assemblies such as fibrillar collagen, the non‐linear process called second harmonic generation (SHG) has also proven to be an important diagnostic tool for the visualization of ECM compartments in...