Comparison of the effectiveness of four different crosslinking agents with hyaluronic acid hydrogel films for tissue‐culture applications

The effectiveness of four different reagents, glutaraldehyde (GTA), 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC), poly(ethyelene glycol) diglycidyl ether (EX 810), and divinyl sulfone (DVS) as crosslinkers for cast hyaluronic acid (HA) films has been evaluated. Films were prepared by casting from solution and exposed to solutions of the crosslinkers in acetone–water solution. Swelling in water and in phosphate buffered saline (PBS) was...