A novel organotypic culture model of the postnatal mouse retina allows the study of glutamate-mediated excitotoxicity
Abstract
A novel organotypic culture method of mouse retina explants is being introduced and characterized to evaluate its usefulness in studying glutamate excitotoxicity. Retinal whole-mounts were dissected from eyes of C57BL/6 mice aged P10-14 and transferred to poly-D-lysine/laminin coated round coverslips. After 7 days in vitro, retina explants were treated with varying concentrations of L-glutamate and cell death was accessed with TUNEL...
Paper Details
Title
A novel organotypic culture model of the postnatal mouse retina allows the study of glutamate-mediated excitotoxicity
Published Date
Jan 1, 2007
Volume
159
Issue
1
Pages
35 - 42
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