Tracking the in vivo fate of recombinant polypeptides by isotopic labeling
Abstract
We report a method to incorporate a stable isotope (13C) and a radioactive isotope (14C) into a recombinant polypeptide during Escherichia coli culture in M9 minimal medium supplemented with universally labeled 13C- or 14C-labeled glucose. We chose a thermally responsive elastin-like polypeptide (ELP) as a model polypeptide for this study because of its utility in various biotechnology applications such as drug delivery and tissue engineering....
Paper Details
Title
Tracking the in vivo fate of recombinant polypeptides by isotopic labeling
Published Date
Aug 1, 2006
Volume
114
Issue
2
Pages
184 - 192
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