Biochemical modifications of gliadins induced by microbial transglutaminase on wheat flour

Celiac disease (CD) is an immune-mediated disorder caused by the ingestion of wheat gluten. A lifelong, gluten-free diet is required to normalize the intestinal mucosa. We previously found that transamidation by microbial transglutaminase (mTGase) suppressed the gliadin-specific immune response in intestinal T-cell lines from CD patients and in models of gluten sensitivity. SDS-PAGE, Western blot, ELISA, tissue transglutaminase (tTGase) assay...