Effect of insulin-like growth factor-1 (IGF-1) on the gluconeogenesis in calf hepatocytes cultured in vitro.

Published on Mar 1, 2012in Molecular and Cellular Biochemistry3.396
路 DOI :10.1007/S11010-011-1130-9
Jianguo Wang12
Estimated H-index: 12
(JLU: Jilin University),
Xiaoyan Zhu8
Estimated H-index: 8
(JLU: Jilin University)
+ 7 AuthorsGuowen Liu27
Estimated H-index: 27
(JLU: Jilin University)
Sources
Abstract
The major role of insulin-like growth factor-1 (IGF-1) in the liver is to mediate glucose uptake in hepatocytes to synthesize glycogen and maintain blood glucose homeostasis. In this study, to evaluate the role of IGF-1 on gluconeogenesis and nutrient metabolism in dairy cattle, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) expression and enzyme activity were evaluated in primary cultures of bovine hepatocytes treated with different concentrations of IGF-1 by quantitative polymerase chain reaction and spectrophotometry, respectively. The results showed that expression of PC and PEPCK were significantly lower in bovine hepatocytes by IGF-1 treatment in test group compare to the control group (P < 0.01). As IGF-1 concentration increased, PC and PEPCK enzyme activity in bovine hepatocytes decreased. Evaluating PC and PEPCK mRNA levels and enzyme activity may thus be useful to monitor subclinical ketosis in dairy cows.
馃摉 Papers frequently viewed together
4 Authors (Paul W. Caton, ..., Roger Corder)
References23
Newest
#1Zhigang ZhangH-Index: 25
#2Xiaobing Li (JLU: Jilin University)H-Index: 23
Last. Ri-He Zhang (JLU: Jilin University)H-Index: 1
view all 10 authors...
Abstract Primary hepatocytes are commonly used during in vitro studies, but care must be taken with isolation and culture of the cells to ensure their viability. In this study, hepatocytes were isolated from the liver (caudate process) of a newborn calf by the collagenase perfusion and digestion method. The trypan blue exclusion method was used to determine total cell number and the survival rate of hepatocytes, while hepatocyte function was assessed by measuring lactate dehydrogenase, albumin a...
Source
#1Xinwei Li (JLU: Jilin University)H-Index: 34
#2Xiaobing Li (JLU: Jilin University)H-Index: 23
Last. Zhe Wang (JLU: Jilin University)H-Index: 27
view all 9 authors...
Dairy cows experience an increased demand for glucose to support milk production. However, negative energy balance is a common condition in peripartum cows. In response, fat mobilization provides non-esterified fatty acids (NEFAs) for oxidation in the liver to generate ATP. To investigate the effects of NEFAs on gluconeogenesis, the expression and enzyme activity of pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPCK) in cultured bovine hepatocytes were evaluated by quantitative...
Source
#1Mariana Pedernera (USYD: University of Sydney)H-Index: 1
#2Pietro Celi (USYD: University of Sydney)H-Index: 30
Last. W. J. Fulkerson (USYD: University of Sydney)H-Index: 21
view all 6 authors...
The aim of this study was to determine the effect of diet, energy balance and milk production on oxidative stress in early-lactating, Holstein鈥揊riesian dairy cows fed to produce either low or high levels of milk. Indicators of energy balance (non-esterified fatty acids, 尾-hydroxybutyrate, glucose and insulin-like growth factor-1) and indicators of oxidative stress (reactive oxygen metabolites and biological antioxidants) were measured in the first 5聽weeks of lactation. Energy balance indicators ...
Source
#1Golam Kabir (University of Chittagong)H-Index: 3
#2Mosaraf Hossain (University of Chittagong)H-Index: 4
Last. Liaquat AliH-Index: 16
view all 9 authors...
Abstract Aim and Background: Free IGF-1 and IGFBP-1 are associated with obesity which is one of the major features of insulin resistance. But very few studies exist on free IGF-1 and IGFBP-1 in IGT subjects. The present study was undertaken to investigate the association of free IGF-1 and IGFBP-1 with insulin sensitivity in IGT subjects. Subjects and Methods: Ninety-one subjects with impaired glucose tolerance (IGT) were studied along with age- sex- and BMI-matched sixty-one healthy Controls wit...
Source
#1Guo-Min Liu (JLU: Jilin University)H-Index: 4
#3Jiangbin Wang (JLU: Jilin University)H-Index: 6
Last. Xinglin Zhu (JLU: Jilin University)H-Index: 3
view all 6 authors...
Summary The aim of the present study was to compare insulin receptor (IR) gene expression in normal bovine liver ( n =聽7) with samples of liver from cows in the perinatal period with ketosis ( n =聽7) and cows with fatty liver ( n =聽7). Gene expression was determined by internally controlled reverse transcriptase polymerase chain reaction (RT-PCR). The expression of IR mRNA in the liver of ketotic dairy cows was higher than in cows with fatty liver, but in both disease groups the expression was s...
Source
#6David A. Kenny (UCD: University College Dublin)H-Index: 3
Differences in genetic selection criteria for dairy cows internationally have led to divergence in the Holstein-Friesian breed. The objective of this study was to compare hepatic expression of genes of the somatotropic axis in the North American Holstein-Friesian and the New Zealand Holstein-Friesian strains of dairy cow at early and mid lactation. Mature cows of both the North American Holstein-Friesian (n = 10) and New Zealand Holstein-Friesian (n = 10) strains were selected. Liver tissue was ...
Source
#1E. Casta帽eda-Guti茅rrez (Cornell University)H-Index: 6
#2Susanne H. Pelton (Cornell University)H-Index: 8
Last. W.R. Butler (Cornell University)H-Index: 58
view all 4 authors...
Abstract Multiparous Holstein cows ( n =58) were used to study the effects of peripartum dietary supplementation on metabolic status, liver function and reproduction variables. Diets for cows were as follows: (a) no supplementation (CTL), (b) prilled fatty acids as 1.9% of DM (PrFA), (c) calcium salts of long chain n-6 fatty acids as 2.24% of DM (CaLFA) or (d) daily topdressing with 769g of 65% propylene glycol (PGLY). Supplements were fed during the last 21 days before expected calving except f...
Source
#1M.L. Rhoads (MU: University of Missouri)H-Index: 13
Abstract The growth hormone/insulin-like growth factor (IGF) system plays a critical endocrine role controlling nutrient metabolism in dairy cattle. In liver, growth hormone receptor (GHR) and IGF-1 are dynamically regulated by lactation and energy balance. Less is known about the regulation of GHR, IGF-1, and IGF-binding protein mRNA in reproductive tissues (uterus, ovarian follicle, and corpus luteum). The objective was to determine expression patterns for GHR, IGF-1, and IGF-binding protein (...
Source
#1Andr茅e-Anne Dussault (Laval University)H-Index: 6
#2Marc Pouliot (Laval University)H-Index: 27
Real-time polymerase chain reaction (PCR) constitutes a significant improvement over traditional end-point PCR, as it allows the quantification of starting amounts of nucleic acid templates, in real-time. However, quantification requires validation through numerous internal controls and standard curves. We describe in this paper a simple protocol which uses real-time PCR to compare mRNA levels of a gene of interest between different experimental conditions. Comparative real-time PCR can be a rel...
Source
Abstract The objective of this review was to address the basis for and prevention of selected diseases (fatty liver, ketosis, rumen acidosis and displaced abomasums) that are feeding- and management-related and frequent in periparturient cows. The focus has been to discuss (1) the general physiological regulation and adaptations that are believed to be important for a successful transition to lactation, (2) for each disease the disease incidence rate, risk factors, aetiology and pathophysiology ...
Source
Cited By15
Newest
#1Joseph W. McFadden (Cornell University)H-Index: 14
Coordinated changes in energy metabolism develop to support gestation and lactation in the periparturient dairy cow. Maternal physiology involves the partitioning of nutrients (i.e. glucose, amino acids and fatty acids ( FA )) for fetal growth and milk synthesis. However, the inability of the dairy cow to successfully adapt to a productive lactation may trigger metabolic stress characterized by uncontrolled adipose tissue lipolysis and reduced insulin sensitivity. A consequence is lipotoxicity a...
Source
#1Li Dong (Hebei Medical University)H-Index: 1
#2Xiaoyu Hou (Hebei Medical University)H-Index: 1
Last. Guangyao Song (Hebei Medical University)H-Index: 14
view all 7 authors...
: Insulin resistance (IR) is a common etiology of type 2 diabetes (T2D) defined by a state of decreased reactivity to insulin in multiple organs, such as the liver. This study aims to investigate how microRNA-122-5p (miR-122) regulates the hepatic IR in vitro. We first found that the miR-122 level was upregulated in the liver of rats fed with a high-fat diet and injected with streptozotocin (T2D rats), while the expression level of insulin-like growth factor 1 receptor (IGF-1R), a potential targ...
Source
#1Venu Seenappa (Manipal University)H-Index: 4
#2Manjunath B. Joshi (Manipal University)H-Index: 20
Last. Kapaettu Satyamoorthy (Manipal University)H-Index: 46
view all 3 authors...
Background: The phosphoenolpyruvate carboxykinase (PEPCK) isoforms are considered as rate-limiting enzymes for gluconeogenesis and glyceroneogenesis pathways. PEPCK exhibits several interesting features such as a) organelle-specific isoforms (cytosolic and a mitochondrial) in vertebrate clade, b) tissue-specific expression of isoforms and c) organism- specific requirement of ATP or GTP as a cofactor. In higher organisms, PEPCK isoforms are intricately regulated and activated through several phys...
Source
#1Chuanjiang Cai (NWAFU: Northwest A&F University)H-Index: 1
#2Yezi Kong (NWAFU: Northwest A&F University)H-Index: 1
Last. Jianguo Wang (NWAFU: Northwest A&F University)H-Index: 1
view all 4 authors...
The aims of the study reported in the Research Communication were to evaluate changes of macrominerals and major calcitropic hormone concentrations in 51 healthy dairy cows over the peripartum period, and to compare those cows with 51 cows subject to subclinical hypocalcaemia (SCH) on calving day. The results showed that serum calcium (Ca) and potassium (K) concentrations were lowest in the healthy periparturient dairy cows at parturition, while sodium (Na) and parathyroid hormone (PTH) concentr...
Source
Summary Negative energy balance is considered as the pathological basis of energy metabolic disorders in periparturient dairy cows. Serum non-esterified fatty acids (NEFA) are one of the most important indicators of energy balance status. Fibroblast growth factor 21 (FGF21) has been identified as a hepatokine involved in regulation of metabolic adaptations, such as promoting hepatic lipid oxidation and ketogenesis, during energy deprivation. However, the direct effects of NEFA on FGF21 expressio...
Source
#1Rikke Hjortebjerg (OU: Ohio University)H-Index: 13
#2Darlene E. Berryman (OU: Ohio University)H-Index: 28
Last. John J. Kopchick (OU: Ohio University)H-Index: 87
view all 8 authors...
Abstract Growth hormone (GH) is a determinant of glucose homeostasis and adipose tissue (AT) function. Using 7-month-old transgenic mice expressing the bovine growth hormone (bGH) gene and growth hormone receptor knockout (GHR-/-) mice, we examined whether changes in GH action affect glucose, insulin, and pyruvate tolerance and AT expression of proteins involved in the interrelated signaling pathways of GH, insulinlike growth factor 1 (IGF-1), and insulin. Furthermore, we searched for AT depot-s...
Source
#3Bo Zhang (ZJU: Zhejiang University)H-Index: 23
: The objective of the present study was to investigate the nutrient availability for milk production in the mammary gland of lactating cows fed different forage-based diets. The 3 diets contained 30% corn stover (CS), 30% rice straw (RS), or 23% alfalfa hay plus 7% Chinese wild rye hay (AH) as a forage source. All diets contained 15% of DM as corn silage and 55% of DM as concentrate. The percentage of milk lactose was always lower in the RS-fed cows than in the cows fed AH or CS during the 12-w...
Source
#1Igor DzadzovskiH-Index: 3
#2Irena CeleskaH-Index: 5
Last. Danijela KirovskiH-Index: 10
view all 5 authors...
Source
#1Marion PiechottaH-Index: 18
#2W MysegadesH-Index: 1
Last. Heiner Bollwein (UZH: University of Zurich)H-Index: 7
view all 7 authors...
Abstract A study involving a small number of cows found that the concentrations of insulin-like growth hormone 1 (IGF1) may be a useful predictor of metabolic disease. Further, IGF1 may provide also a pathophysiological link to metabolic diseases such as ketosis. The objective of the current study was to test whether the low antepartal total IGF1 or IGF1 binding protein (IGFBP) concentrations might predict ketosis under field conditions. Clinical examinations and blood sampling were performed an...
Source
#1Jianguo Wang (NWAFU: Northwest A&F University)H-Index: 12
#2Xiaoyan Zhu (French Institute of Health and Medical Research)H-Index: 1
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 6 authors...
An elevated plasma non-esterified fatty acids (NEFA) concentration is an important indicator of negative energy balance, which is often associated with metabolic diseases such as fatty liver and ketosis. Angiopoietin-like protein 4 (ANGPTL4) is a hepatokine that plays important roles in the regulation of lipid metabolism. However, the direct effects of high concentrations of NEFA on ANGPTL4 expression and secretion in hepatocytes from cows are not entirely clear. The objective of this study was ...
Source
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.