Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering

A novel method for the preparation of gelatin scaffolds was designed by varying the crosslinking temperature. Four pore size ranges of genipin-crosslinked gelatin scaffolds were made by varying the crosslinking temperature from 10 to 25 degrees C, with the pore sizes ranging from 50 to 500 microm. The pore size of the scaffold increases as the crosslinking temperature increases. Articular chondrocytes of Wistar rats were in vitro cultured in...