ELEVATED GLUCOSE AND FATTY ACID LEVELS IMPAIR SUBSTANCE P-INDUCED DERMAL MICROVASCULAR ENDOTHELIAL CELL MIGRATION AND PROLIFERATION IN AN AGAROSE GEL MODEL SYSTEM

Substance P (SP), a sensory nerve derived neuropeptide, has been implicated in wound repair. Our hypothesis was that oxidative effects of elevated glucose and fatty acid levels as seen with diabetes mellitus inhibit SP-mediated endothelial cell directional migration and proliferation. Using a 2% agarose gel, immortalized human microvascular endothelial cells (HMEC-1) were plated into a 1.5-mm well, and agonist (SP; 10(-4) mol/L) was loaded into...