Self-Assembly of Enzymes on DNA Scaffolds: En Route to Biocatalytic Cascades and the Synthesis of Metallic Nanowires
Abstract
DNA strands consisting of programmed sequence-specific domains were synthesized by the rolling circle amplification (RCA) process. The spatial positioning of glucose oxidase (GOx) and of horseradish peroxidase (HRP) on the RCA-synthesized DNA template via hybridization enabled the activation of the bienzyme cascade. The GOx-catalyzed oxidation of glucose yielded gluconic acid and H2O2, and the resulting H2O2 oxidized...
Paper Details
Title
Self-Assembly of Enzymes on DNA Scaffolds: En Route to Biocatalytic Cascades and the Synthesis of Metallic Nanowires
DOI
Published Date
Mar 26, 2009
Journal
Volume
9
Issue
5
Pages
2040 - 2043
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Notes
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