A colloidal gold probe-based silver enhancement immunochromatographic assay for the rapid detection of abrin-a

High-affinity anti-abrin-a monoclonal and polyclonal antibodies were used to develop a sandwich immunochromatographic assay and silver enhancement technology was used to further increase the sensitivity. Using a matrix of double distilled water or soybean milk with added abrin-a, the visual detection limit was found to be 10 ng mL−1. The detection limit was 0.1 ng mL−1 for abrin-a, an increase in sensitivity of 100-fold when the silver...