Binding of antiphospholipid antibodies to discontinuous epitopes on domain I of human β₂‐glycoprotein I: Mutation studies including residues R39 to R43

Objective Pathogenic antiphospholipid antibodies (aPL) bind the self antigen N‐terminal domain (domain I) of β 2 ‐glycoprotein I (β 2 GPI), with residues G40–R43 being important. However, peptides homologous to other regions of domain I have also been shown to bind aPL. Furthermore, there are no published reports of the effects of altering R39, which has greater surface exposure than the G40–R43 residues. Methods We used a novel, efficient...