Fabrication of poly(L-glutamic acid)/chitosan polyelectrolyte complex porous scaffolds for tissue engineering

Published on Feb 20, 2013in Journal of Materials Chemistry B5.344
· DOI :10.1039/C2TB00440B
Shifeng Yan22
Estimated H-index: 22
(SHU: Shanghai University),
Kunxi Zhang14
Estimated H-index: 14
(SHU: Shanghai University)
+ 6 AuthorsJingbo Yin24
Estimated H-index: 24
(SHU: Shanghai University)
Sources
Abstract
Porous scaffolds composed of polypeptides and polysaccharides have remarkable biocompatibility and potential to mimic an extracellular matrix for tissue engineering. This study presented a novel design of polyelectrolyte complex porous scaffolds of a synthetic polypeptide poly(L-glutamic acid) (PLGA) and a natural polysaccharide chitosan (CS) using a freeze drying method. The microstructure of the porous scaffolds could be adjusted by changing the freezing temperature and solid content of the reacting polymer. PLGA/CS scaffolds fabricated from 2% solid content and at a freezing temperature of −20 °C exhibited an interconnected porous structure with average pore size between 150 and 200 μm. The contact angle of less than 75° and high swelling ratio of more than 700% showed the excellent hydrophilic performance of these scaffolds. Degradation of the PLGA/CS composite scaffolds could be modified and more CS content contributed a higher resistance to biodegradation. The mechanical properties of the scaffolds could be controlled by varying the PLGA/CS molar ratio and solid content. The scaffolds exhibited good elastic behavior in wet state. In vitro culture of rabbit adipose-derived stem cells (ASCs) indicated that the selected PLGA/CS porous scaffolds supported cell attachment and growth. In summary, the PLGA/CS porous scaffolds show excellent properties, such as an interconnected porous structure, mechanical strength, hydrophilicity, biodegradability and biocompatibility. The successful repair of articular cartilage defects showed the potentiality of using PLGA/CS scaffolds in cartilage tissue engineering.
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