Eluotropic strength of solvents

The importance of solvent strength in solvent optimization for reversed-phase high-performance liquid chromatography (RP-HPLC) is discussed. Several “strength” parameters are reviewed and their limitations and accuracy of predicting isoeluotropic solvent compositions are shown. Partition coefficient of the solvent is suggested as a solvent strength parameter. Its use resulted in relatively precise prediction of mobile phase compositions which...