Novel LC–MS/MS method for analyzing imperialine in rat plasma: Development, validation, and application to pharmacokinetics

A sensitive, selective, rapid liquid chromatography–electrospray ionization-tandem mass spectrometric method was developed and validated in rat plasma to quantify imperialine, a major active constituent extracted from Bulbus Fritillariae Cirrhosae. Before analysis, plasma samples were pre-treated using cost-effective protein precipitation in order to extract imperialine and the internal standard, carbamzepine. The two analytes were then...