Culture of bovine hepatocytes: a non-perfusion technique for cell isolation
Abstract
In this work we have studied the isolation and culture of mature bovine hepatocytes on plastic dishes without exogenous matrix. The liver has been disaggregated in a collagenase solution instead of undergoing a perfusion step. After a few days in culture, the plates showed several clusters of different cell types. Although the average yield was 1.60±0.57×108 viable liver cells per gram of tissue, these cultures were formed by non-parenchymal...
Paper Details
Title
Culture of bovine hepatocytes: a non-perfusion technique for cell isolation
Published Date
Sep 5, 2006
Journal
Volume
51
Issue
2
Pages
51 - 56
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History