Modulation Of Sulfur Mustard-induced Inflammation And Gene Expression By Olvanil In The Hairless Mouse Vesicant Model

Published on Jun 30, 2003in Journal of Toxicology-cutaneous and Ocular Toxicology
· DOI :10.1081/CUS-120022753
Carol L. K. Sabourin24
Estimated H-index: 24
(Battelle Memorial Institute),
Michele M. Danne2
Estimated H-index: 2
(Battelle Memorial Institute)
+ 5 AuthorsRobert P. Casillas20
Estimated H-index: 20
(United States Army Medical Research Institute of Chemical Defense)
Sources
Abstract
Cutaneous exposure to sulfur mustard [bis(2-chloroethyl) sulfide (SM)] produces a delayed inflammatory skin response that is followed by severe dermal injury. Assessment of anti-inflammatory therapies against SM-induced skin injury has mainly relied on qualitative histopathological evaluation. The goal of this study was to identify proinflammatory biomarkers in the hairless mouse vesicant model that could be used as additional indicators of SM-induced skin injury for evaluating anti-inflammatory treatment. SM-induced inflammation was determined at 2, 6, and 24 hr postexposure by changes in edema. Ribonuclease protection assay (RPA) was used to determine changes in gene expression of inflammatory mediators. At 2, 6, and 24 hr postexposure, a time-dependent increase in edema was observed in SM-exposed skin, which was significant at 6 and 24 hr when compared to unexposed controls. Ribonuclease protection assay analysis revealed a two-fold or greater increase in monocyte chemoattractant protein-1 (MCP-1), mac...
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