Size-exclusion chromatography dimension for rod-like macromolecules

A phenomenological approach has been taken to determine the macromolecular dimension that controls peak migration in size-exclusion chromatography. For macromolecules representative of the class of rigid rods, flexible rods, random coils, and compact ellipsoids, the dependence of the chromatographic partition coefficient, KSEC, on the viscosity radius, the radius of gyration, and the contour length, respectively, was determined. Since none of...