Hapten-Functionalized DNA-Streptavidin Nanocircles as Supramolecular Reagents in a Competitive Immuno-PCR Assay

Analysis with nanorings: The endogeneous proteins of supramolecular DNA nanocircles, obtained in high yields from oligomeric precursors containing bisbiotinylated DNA and streptavidin, are conveniently functionalized with biotinylated hapten moieties (see schematic representation). These modular conjugates can be used as reagents in a novel competitive immunoassay for the high-sensitivity detection of low molecular weight...