Protein Thioester Synthesis Enabled by Sortase

Volume: 134, Issue: 26, Pages: 10749 - 10752
Published: Jun 19, 2012
Abstract
Proteins containing a C-terminal thioester are important intermediates in semisynthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. Here we report a simple strategy, utilizing sortase A, for routine preparation of recombinant proteins containing a C-terminal (α)thioester. We used our method to prepare two different anthrax toxin cargo proteins: one containing an...
Paper Details
Title
Protein Thioester Synthesis Enabled by Sortase
Published Date
Jun 19, 2012
Volume
134
Issue
26
Pages
10749 - 10752
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