Isolation and characterization of human mesenchymal stem cells from gingival connective tissue

Background and Objective The main purpose of this study was to isolate and characterize gingival connective tissue‐derived mesenchymal stem cells ( GMSC s). The secondary purpose was to present a modified isolation method for the GMSC s. Material and Methods Collected healthy gingival tissue samples were de‐epithelialized and minced into small fragments. The tissues were digested by dispase and collagenase IV for 30 min. The first digested cell...