Comparison of Vitreolytic Effect in Rabbit Eyes: Plasmin, Hyaluronidase, and Their Mixtures

Published on Jun 1, 2009in Journal of The Korean Ophthalmological Society
· DOI :10.3341/JKOS.2009.50.6.911
Moo Sang Kim4
Estimated H-index: 4
,
Sang Woong Moon16
Estimated H-index: 16
(Inje University)
+ 2 AuthorsHyung Woo Kwak14
Estimated H-index: 14
Sources
Abstract
Purpose: The aim of the present study was to quantify and compare the vitreolytic effect of plasmin, hyaluronidase, and a combination of the two. Methods: Thirty-six rabbits were randomized into 3 groups: (A) twelve rabbits had an intravitreal injection of plasmin 1 U with hyaluronidase 10 U/0.1 mL into the right eye, (B) twelve rabbits had an injection of plasmin alone (1 U/0.1 mL), and (C) twelve rabbits had an injection of hyaluronidase alone (10 U/0.1 mL). The left eye of each rabbit was used as control, which was injected with 0.1 mL phosphate buffered saline (PBS). The eyes were enucleated 1 hour and 24 hours after injection. The volume of fluid-type vitreous and gel-type vitreous was measured with a micropipette using the melting point as the difference. Statistical analysis was performed and light microscopy was used to assess potential damage to the retinal tissue. Results: The volume of remaining gel-type vitreous was measured as 52.5%, 60.3%, 59.2%, and 76.5% after 1 hour enucleation and as 44.6%, 56.7%, 56.1%, and 74.7%, after 24 hours enucleation in group A, B, C, and control group, respectively. Group A, B, and C showed statistically significant differences against the control group. Group A (plasmin with hyaluronidase) showed less remaining gel-type vitreous volume than a single injection of plasmin or hyaluronidase alone. Conclusions: Intravitreal injection of plasmin with hyaluronidase showed more vitreolytic effect than a single injection of plasmin or hyaluronidase alone. The enzyme may be useful in liquefying the vitreous, and may be a useful biochemical adjunct to vitrectomy.
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References26
Newest
#1Dong Su Kim (Inje University)H-Index: 2
#2Sang Woong Moon (Inje University)H-Index: 16
Last. Hyung Woo Kwak (Kyung Hee University)H-Index: 14
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Purpose: This study evaluated the effect of tissue plasminogen activator (TPA) on adhesion between the internal limiting membrane and the vitreous cortex in patients with diabetic macular edema. Methods: This retrospective study included 14 eyes of 14 patients with diabetic macular edema without posterior vitreous detachment. The fourteen eyes were divided into two groups. In the first group, vitrectomy and internal limiting membrane (ILM) removal were performed after intravitreal TPA 0.1 cc (50...
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#1Baruch D. Kuppermann (UCI: University of California, Irvine)H-Index: 57
#2Edgar L. ThomasH-Index: 11
Last. L.R. GrilloneH-Index: 6
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Purpose To evaluate the efficacy of intravitreous ovine hyaluronidase for the management of vitreous hemorrhage. Design Two prospective, randomized, placebo-controlled, double-masked studies. Safety data are presented in a companion article in the journal. Methods Eligible patients with vitreous hemorrhage ≥1 month duration; severe at entry with best corrected visual acuity (BCVA) worse than 20/200 were randomized to 55 IU or 75 IU ovine hyaluronidase or saline. Primary efficacy (clearance of he...
Source
#1Baruch D. Kuppermann (UCI: University of California, Irvine)H-Index: 57
#2Edgar L. ThomasH-Index: 11
Last. L.R. GrilloneH-Index: 6
view all 4 authors...
Purpose To evaluate the safety of intravitreous ovine hyaluronidase for the management of vitreous hemorrhage. Design Two prospective, randomized, placebo-controlled, double-masked studies. Pooled efficacy data are presented in a companion article in this issue of THE JOURNAL. Methods Subjects with vitreous hemorrhage ≥1 month, severe at entry and best corrected visual acuity (BCVA) worse than 20/200 in the study eye were randomized to 7.5 IU, 55 IU, 75 IU ovine hyaluronidase, saline, or no inje...
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Source
To study whether intravitreal injection of plasmin + hyaluronidase safely induces posterior vitreous detachment (PVD). Rabbits were randomized into three groups: (A) 20 rabbits, intravitreal injection of plasmin 1 U + hyaluronidase 20 U in balanced salt solution (BSS) 0.1 mL into one eye; (B) 12 rabbits, plasmin alone; (C) 12 rabbits, hyaluronidase alone. The fellow eye of each rabbit was injected BSS 0.1 mL. In Group A, scanning electron microscopy (SEM) was done in four rabbits at 0.5 hour and...
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#1Flemming Staubach (University of Freiburg)H-Index: 6
#2Veronika Nober (University of Freiburg)H-Index: 1
Last. P. Janknecht (University of Freiburg)H-Index: 10
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Purpose. Facilitation of vitrectomy by vitreolytic enzymes may be of great value in complicated or office-procedure vitreo-retinal surgery. In this study, we quantified and compared the effect of hyaluronidase, chondroitinase, and plasmin pre-incubation on vitrectomy rate and explored potential retinal damage. Methods. Freshly enucleated pigs eyes were incubated (1 or 3 hours) with an intravitreally injected enzyme or control solution. Enzyme doses were 100 and 1000 U for hyaluronidase, 1 and 2 ...
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PURPOSE. To evaluate the safety and efficacy of dispase and plasmin when inducing posterior vitreous detachment (PVD) by intravitreous injection in rabbit eyes. METHODS. Forty-eight young pigmented rabbits were randomized into six groups. Groups 1 and 5 received 0.025 U dispase in test eyes; group 2, 0.1 U dispase; groups 3 and 6, 1 U plasmin; and group 4, 4 U plasmin. All groups received PBS in control eyes. Groups 5 and 6 were euthanatized 15 minutes after surgery for ocular histologic examina...
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#1Sohan Singh Hayreh (UI: University of Iowa)H-Index: 113
#2Jost B. JonasH-Index: 153
Background: To evaluate the frequency of, and the factors which are primarily and secondarily associated with, posterior vitreous detachment (PVD), and to correlate the presence of
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#1Namju Kim (SNU: Seoul National University)H-Index: 15
#2Hyeong Gon Yu (SNU: Seoul National University)H-Index: 32
Last. Hum Chung (SNU: Seoul National University)H-Index: 34
view all 4 authors...
prime purposes of vitrectomy. Despite the much improved surgical techniques and equipment, the complete separation of the vitreous cortex from the internal limiting membrane (ILM) remains difficult. Moreover, the mechanical peeling of the hyaloid from the retinal surface may be associated with the risk of iatrogenic retinal breaks and hemorrhages.1 For some time, vitreoretinal surgeons have requested minimally invasive vitreolytic solutions for surgical problems in cases of vitreoretinal disease...
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#1Koh Hei Sonoda (Kyushu University)H-Index: 39
#2Taiji Sakamoto (Kyushu University)H-Index: 61
Last. Tatsuro Ishibashi (Kyushu University)H-Index: 80
view all 10 authors...
Abstract Objective To visualize the residual vitreous cortex (VC) on the retinal surface after surgical posterior vitreous separation (PVS) during a pars plana vitrectomy (PPV), especially in patients with diabetic retinopathy. Design Case–control study. Participants Patients with proliferative diabetic retinopathy (PDR), diabetic macular edema (DME), branch retinal vein occlusion (BRVO), and rhegmatogenous retinal detachment (RRD). Methods A triamcinolone acetonide (TA)–assisted vitrectomy was ...
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Cited By1
Newest
#1Chaerin Park (New Generation University College)
#2Sun Ho Lee (Jeju National University)H-Index: 2
Last. Hum Chung (SNU: Seoul National University)H-Index: 34
view all 4 authors...
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