SEC determination of cross-link efficiency in hyaluronan fillers

The cross-link efficiency in hyaluronan fillers was determined by means of triple detector (RI, LALS-RALS and a Differential Viscometer) GPC/SEC in aqueous buffer. The low water solubility of HA cross-linked with BDDE (HBC) was overcome with an alkaline hydrolysis step. The kinetics of linear HA hydrolysis was investigated at different NaOH concentrations (0.25–0.5–1 M), initial polymer Mws (200–700–1000–1200–1600 kDa) and polymer concentrations...