Assembly of nucleic acid-lipid nanoparticles from aqueous-organic monophases

Effective, reproducible, and scalable methods for DNA–lipid assembly are important for the success of non-viral vectors in in vivo gene therapy. We hypothesized DNA–lipid assembly would be optimal if started from a liquid monophase where both DNA and lipids separately form molecular or micellar solutions prior to mixing, without preexisting condensed lipid phases, thus allowing DNA–lipid assembly under conditions close to equilibrium....