Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag
Abstract
We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O6-benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be used to label subcellular structures in Zebrafish such as the nucleus, cell membranes, and endosomal membranes. The stability of the synthetic fluorophores makes them attractive choices for...
Paper Details
Title
Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag
Published Date
Feb 28, 2011
Journal
Volume
240
Issue
4
Pages
820 - 827
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