Discrimination of the seeds of Notopterygium incisum and Notopterygium franchetii by validated HPLC-DAD–ESI-MS method and principal component analysis

A validated HPLC-DAD–ESI-MS method has been developed to simultaneously quantify 12 bioactive compounds in the seeds of Notopterygium incisum Ting ex H.T. Chang and Notopterygium franchetii H. de Boiss whose rhizomes and roots are widely used as traditional Chinese medicine. This method was validated to be sensitive, precise and accurate and was applied to evaluate the difference in the chemical profiles and contents of these analytes in 37...