Effects of Traditional Chinese Medicinal Plants on Anti-insulin Resistance Bioactivity of DXMS-Induced Insulin Resistant HepG2 Cells.

Published on Jul 10, 2014in Natural Products and Bioprospecting
· DOI :10.1007/S13659-014-0028-0
Jun-Zeng Ma7
Estimated H-index: 7
(CAS: Chinese Academy of Sciences),
Lixin Yang5
Estimated H-index: 5
(CAS: Chinese Academy of Sciences)
+ 7 AuthorsGang Xu36
Estimated H-index: 36
(CAS: Chinese Academy of Sciences)
Medicinal plants have a long history of use in China to treat diabetic symptoms. Ancient Chinese medical manuscripts and ethnobotanical surveys document plant remedies that continue to be actively used in China for the treatment of diabetic symptoms. Based on a systematic ancient Chinese medical manuscripts review in combination with ethnobotanical survey, 16 medicinal plants for the traditional treatment of diabetic symptoms were identified for the evaluation of anti-insulin resistance bioactivity. The biological activity of 16 medicinal plants was tested on dexameth- asone (DXMS)-induced insulin resistant HepG2 cells. The result shows that 11 of the 16 medicinal plants enhanced glucose uptake of DXMS-induced insulin resistant HepG2 cells, thereby demonstrating their ability to increase insulin sensitivity, other five medicinal plants including Astragalus membranaceus were found ineffective. The study shows that ancient Chinese medical manuscripts and ethnobotanical surveys on plants for the prevention and treatment of diabetic symptoms provide a promising knowledge base for drug discovery to mitigate the global diabetes epidemic.
#1Ka Wu (Guangxi Medical University)H-Index: 1
#2Tao Liang (Guangxi Medical University)H-Index: 1
Last. Rong Li (GLMU: Guilin Medical University)H-Index: 10
view all 6 authors...
Abstract Diabetes is a chronic disease characterized by the metabolic disorder in specific tissues. Our present study was designed to assess the potential benefits of puerarin (PR) on hypoglycemic and hypolipemic effects in diabetic mice induced by streptozotocin (STZ). The results achieved from these experiments showed that glycemia in STZ-diabetogenic mice were significantly reduced following the PR administration, while serum insulin concentration was increased. In addition, PR contributed to...
54 CitationsSource
#1Chao Guo (Guangxi Medical University)H-Index: 8
#2Rong Li (GLMU: Guilin Medical University)H-Index: 10
Last. Qiaoling He (Guangxi Medical University)H-Index: 7
view all 6 authors...
Abstract Diabetes mellitus is a clinically complex disease characterized by the dysfunctions of pancreas. In this study, we investigated the therapeutic effects of ramulus mori polysaccharides (RMP) on diabetic mice induced by streptozotocin. Our results showed that body weight and insulin level were notably increased after metformin and RMP treatments, while the blood glucose was lowered. HE-staining assay showed that the treatments mitigated the pathological lesions in pancreas tissue. In addi...
41 CitationsSource
#1Susan CostantiniH-Index: 33
#2G. Di Bernardo (Seconda Università degli Studi di Napoli)H-Index: 13
Last. Giovanni Colonna (Seconda Università degli Studi di Napoli)H-Index: 31
view all 5 authors...
Abstract Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and is associated with various clinico-pathological characteristics such as genetic mutations and viral infections. Therefore, numerous laboratories look out for identifying always new putative markers for the improvement of HCC diagnosis/prognosis. Many molecular profiling studies investigated gene expression changes related to HCC. HepG2 represents a pure cell line of human liver carcinoma, often used as HCC mode...
65 CitationsSource
#1Shuang Jiang (JLU: Jilin University)H-Index: 1
#2Peige Du (Beihua University)H-Index: 7
Last. Zhiwei Sun (Capital Medical University)H-Index: 1
view all 5 authors...
Abstract For the past few years, numerous polysaccharides and polysaccharide-protein complexes have been isolated from plant or animal and used as a promising source of therapeutic agents for diabetes mellitus (DM). In this study, a water-soluble polysaccharide, named as CCPW-1, was extracted and fractioned from the roots of Coptis Chinensis by DEAE Sepharose Fast Flow anion-exchange and Sephadex G-100 column chromatography. The determination of the monosaccharide composition in CCPW-1 with gas ...
47 CitationsSource
#1Sheng-Zi Liu (Hunan Normal University)H-Index: 2
#2Yuan-Xiong Deng (Hunan Normal University)H-Index: 7
Last. Xi-Min Qiu (Hunan Normal University)H-Index: 5
view all 6 authors...
Abstract Ethnopharmacological relevance Scutellaria–coptis herb couple (SC) is the main herb couple in many traditional Chinese compound formulas used for the treatment of diabetes mellitus, which has been used to treat diabetes mellitus for thousands of years in China. In this study we provide experimental evidence for the clinical use of SC in the treatment of diabetes mellitus. Aim of the study To confirm the anti-diabetic effect of SC extract and its main components, and to explore its mecha...
29 CitationsSource
#1Eun-Kyung AhnH-Index: 8
#1Eun-Kyung AhnH-Index: 9
Last. Joa Sub Oh (DU: Dankook University)H-Index: 11
view all 5 authors...
: In order to determine anti-adipogenic effect, this study investigated 1β-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding pr...
20 CitationsSource
#1Yongkui Liu (Chang'an University)H-Index: 17
#2Xiaojie Chen (IIASA: International Institute for Applied Systems Analysis)H-Index: 31
Last. Matjaž Perc (University of Maribor)H-Index: 93
view all 5 authors...
Holding on to one's strategy is natural and common if the later warrants success and satisfaction. This goes against widespread simulation practices of evolutionary games, where players frequently consider changing their strategy even though their payoffs may be marginally different than those of the other players. Inspired by this observation, we introduce an aspiration-based win-stay-lose-learn strategy updating rule into the spatial prisoner's dilemma game. The rule is simple and intuitive, f...
453 CitationsSource
#1Kai He (SWU: Southwest University)H-Index: 16
#2Xuegang Li (SWU: Southwest University)H-Index: 3
Last. Hejing Shu (SWU: Southwest University)H-Index: 1
view all 11 authors...
Abstract Aims Traditional Chinese medicine (TCM) has been used for treating complex chronic diseases owing to their fewer side-effects, better patient tolerance and relatively less cost. The present work was carried out to study the anti-diabetic efficacy and mechanisms of 34 TCMs. Materials and methods Streptozotocin (STZ)-diabetic mice were orally administrated with corresponding herbal solution once a day for 4 weeks. At the end of experiment, the level of plasma glucose, malondialdehyde (MDA...
85 CitationsSource
#1Clare F. PriceH-Index: 5
#2David Tyssen (Burnet Institute)H-Index: 14
Last. Gilda Tachedjian (Monash University)H-Index: 36
view all 13 authors...
SPL7013 Gel (VivaGel®) is a microbicide in development for prevention of HIV and HSV. This clinical study assessed retention and duration of antiviral activity following vaginal administration of 3% SPL7013 Gel in healthy women. Participants received 5 single doses of product with ≥5 days between doses. A cervicovaginal fluid (CVF) sample was collected using a SoftCup™ pre-dose, and immediately, or 1, 3, 12 or 24 h post-dose. HIV-1 and HSV-2 antiviral activities of CVF samples were determined in...
112 CitationsSource
#1Wenyi Kang (Henan University)H-Index: 4
#2Yanli Song (Henan University)H-Index: 2
Last. Li Zhang (Henan University)H-Index: 2
view all 3 authors...
Different solvent extracts of Hypericum ascyron L. were evaluated by α-glucosidase inhibitory activity and DPPH, ABTS and FRAP assays, respectively, for antioxidant properties in vitro. EtOAC and MeOH extracts showed stronger inhibiting activity against α-glucosidase and rat intestinal α-glucosidase compared with acarbose as positive control. Eight known compounds were isolated from EtOAC and MeOH extracts and screened for α-glucosidase inhibitory and antioxidant activities. Kaempferol and ursol...
46 CitationsSource
Cited By7
#1Shazia Noureen (UOS: University of Sargodha)H-Index: 2
#2Sobia Noreen (UOS: University of Sargodha)H-Index: 8
Last. Syed Nasir Abbas BukhariH-Index: 28
view all 5 authors...
Cuscuta, commonly known as dodder, is a genus of family convolvolaceace. Approximately 170 species of Cuscuta are extensively distributed in temperate and subtropical areas of the world. Species of this genus are widely used as essential constituents in functional foods and traditional medicinal systems. Various parts of many members of Cuscuta have been found efficacious against a variety of diseases. Phytochemical investigations have confirmed presence of biologically active moieties such as f...
3 CitationsSource
Purpose: The aim of this study was to determine whether resveratrol (Rev) affects the expression, phosphorylation, and nuclear and cytoplasmic distribution of histone deacetylase 4 (HDAC4), which in turn affects gluconeogenesis in hepatocytes under an insulin-resistant state. Materials and methods: HepG2 cells were treated with 0.25 mmol/L palmitic acid (PA) to establish an insulin resistance model. The cells were divided into five groups: control, PA, PA + Rev 100 µM, PA + Rev 50 µM, and PA + R...
5 CitationsSource
#1Pardeep Kaur (G.N.D.U.: Guru Nanak Dev University)H-Index: 7
#2Robin (G.N.D.U.: Guru Nanak Dev University)H-Index: 4
Last. Balbir Singh (G.N.D.U.: Guru Nanak Dev University)H-Index: 9
view all 5 authors...
Cancer cell lines of human tissue origin have been extensively used to investigate antiproliferative activity and toxicity of herbal extracts, isolated compounds, and anticancer drugs. These cell lines are genetically and/or epigenetically well characterized to determine the altered expression of proteins within given cellular pathways and critical genes in cancer. Human derived hepatoma (HepG2) cell line has been extensively exploited to examine cytoprotective, antioxidative, hepatoprotective, ...
3 CitationsSource
#1Yamin BibiH-Index: 10
#2Muhammad ArshadH-Index: 43
Last. Sunbal Khalil ChaudhariH-Index: 6
view all 6 authors...
Polygonatum verticillatum (Linn.) All. syn. Convallaria verticillata Linn. is a valuable medicinal plant, distributed in the temperate Himalaya at the elevations 2 400 to 2 800 m. It is a perennial rhizomatous herb and contains various pharmacologically important secondary metabolites among which the most important are α-bulnesene, linalyl acetate, eicosadienoic, pentacosane, piperitone, docasane, diosgenin, santonin and calarene. It also possesses antimalarial, antipyretic, anti-inflammatory, a...
4 CitationsSource
#1Xiao-Tian Zhang (MOE: Chinese Ministry of Education)H-Index: 1
#2Chun-Jiang YuH-Index: 1
Last. Fang Wang (MOE: Chinese Ministry of Education)H-Index: 1
view all 10 authors...
We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P <...
1 CitationsSource