qPCR assay for detecting and quantifying a virulence plasmid in acute hepatopancreatic necrosis disease (AHPND) due to pathogenic Vibrio parahaemolyticus

Volume: 442, Pages: 12 - 15
Published: May 1, 2015
Abstract
null null A quantitative polymerase chain reaction (qPCR) assay was developed, based on a TaqMan probe, to detect and quantify a virulence plasmid harbored by the bacterium null Vibrio parahaemolyticus null which can cause acute hepatopancreatic necrosis disease (AHPND). The assay uses a pair of PCR primers, which amplify a 135-bp DNA fragment, and a TaqMan probe selected from the plasmid null pir A-like gene. This qPCR assay reacted with...
Paper Details
Title
qPCR assay for detecting and quantifying a virulence plasmid in acute hepatopancreatic necrosis disease (AHPND) due to pathogenic Vibrio parahaemolyticus
Published Date
May 1, 2015
Volume
442
Pages
12 - 15
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