Construction of Sandwich Enzyme Immunoassay for Rat Transthyretin Using Recombinant Antigen Approach, and Its Application

Volume: 57, Issue: 3, Pages: 414 - 418
Published: Mar 23, 1993
Abstract
A sandwich enzyme immunoassay for rat transthyretin using a recombinant antigen approach is described. Rat transthyretin cDNA was cloned by PCR. Rat transthyretin fused with the IgG-binding ZZ domain of protein A was expressed in E. coli using the fusion vector pEZZ18. Antibody against the fusion protein was specific to rat transthyretin in plasma as shown by immunoblotting. Affinity-purified anti-rat transthyretin-ZZ was biotinylated and used...
Paper Details
Title
Construction of Sandwich Enzyme Immunoassay for Rat Transthyretin Using Recombinant Antigen Approach, and Its Application
Published Date
Mar 23, 1993
Volume
57
Issue
3
Pages
414 - 418
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