Liquid chromatography–tandem mass spectrometry analysis of metabolites in rats after administration of prenylflavonoids from Epimediums

The metabolites in rats after administration of icariside II, icariin, epimedin C and extracts of four Epimedium species were investigated. Feces, bile, plasma and urine samples were detected comprehensively using HPLC-ESI-MS(n) method. The structures of metabolites were identified on the basis of their characteristic fragmentations in MS(n) experiments. Totally, 54 metabolites were identified in these biosamples. Specific hydrolysis of 7-O...