A sensitive enzyme assay for biotin, avidin, and streptavidin
Abstract
Reciprocal enzyme assays are described for the vitamin biotin and for the biotin-binding proteins avidin and streptavidin. The assays are based on the following steps: (a) biotinylated bovine serum albumin is adsorbed onto microtiter plates; (b) streptavidin (or avidin) is bound to the biotin-coated plates; (c) biotinylated enzyme (in this case alkaline phosphatase) is then interacted with the free biotin-binding sites on the immobilized...
Paper Details
Title
A sensitive enzyme assay for biotin, avidin, and streptavidin
Published Date
Apr 1, 1986
Journal
Volume
154
Issue
1
Pages
367 - 370
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