Matrix mineralization in MC3T3-E1 cell cultures initiated by β-glycerophosphate pulse

MC3T3-E1 cells, grown in the presence of serum and ascorbate, express alkaline phosphatase and produce an extensive collagenous extracellular matrix that can be mineralized by the addition of β-glycerophosphate (β-GP). In the present work, we study the influence of concentration and duration of β-GP treatment on the mineralization pattern in 4-week-old cell cultures. Amount and structure of mineral deposition were monitored by von Kossa...