Raft-like membranes from the trans-Golgi network and endosomal compartments

Volume: 8, Issue: 12, Pages: 2429 - 2439
Published: Nov 7, 2013
Abstract
This article describes a procedure to prepare a raft-like intracellular membrane fraction enriched for the trans-Golgi network (TGN) and endosomal compartments. The initial step in this technique involves cell disruption by homogenization, followed by clearance of the plasma membrane, late endosomes, mitochondria and the endoplasmic reticulum by differential sedimentation. Carbonate treatment, sonication and sucrose density-gradient...
Paper Details
Title
Raft-like membranes from the trans-Golgi network and endosomal compartments
Published Date
Nov 7, 2013
Volume
8
Issue
12
Pages
2429 - 2439
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