The determination and prevention of cytotoxic effects induced in human lymphocytes by the alkylating agent 2,2′-dichlorodiethyl sulfide (sulfur mustard, HD)

2,2′-Dichlorodiethyl sulfide (sulfur mustard, HD, 1,1′-thiobis[2-chloroethane]) is a potent vesicant which can cause severe lesions to skin, lung, and eyes. There is no convenient in vitro or in vivo method(s) to objectively measure the damage induced by HD; therefore, a simple in vitro method was developed using human peripheral lymphocytes to study HD-induced cytotoxicity. The cytotoxicity of HD was measured using dye exclusion as an indicator...