Protocols for finding the most orthogonal dimensions for two-dimensional high performance liquid chromatography
Abstract
The selection of two high performance liquid chromatography (HPLC) columns with vastly different retention mechanisms is vital for performing effective two-dimensional (2D-) HPLC. This paper reports on a systematic method to select a pair of HPLC columns that provide the most different separations for a given sample. This was completed with the aid of a HPLC simulator that predicted retention profiles on the basis of real experimental data,...
Paper Details
Title
Protocols for finding the most orthogonal dimensions for two-dimensional high performance liquid chromatography
Published Date
Mar 1, 2015
Journal
Volume
134
Pages
402 - 408
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