A Sensitive LC–MS–MS Method for Simultaneous Quantification of Two Structural Isomers, Hyperoside and Isoquercitrin: Application to Pharmacokinetic Studies

We report the development and validation of a rapid, specific, and sensitive liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method for analysis and pharmacokinetic study, in rats, of hyperoside and isoquercitrin, two bioactive structural isomers present in the leaves of Apocynum venetum L. After simple deproteinization by addition of acetonitrile, the analytes were separated on a C18 column. Detection was by tandem mass spectrometry...