Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells
Abstract
Mass spectrometry (MS)-based proteomics typically employs multistep sample-preparation workflows that are subject to sample contamination and loss. We report an in-StageTip method for performing sample processing, from cell lysis through elution of purified peptides, in a single, enclosed volume. This robust and scalable method largely eliminates contamination or loss. Peptides can be eluted in several fractions or in one step for single-run...
Paper Details
Title
Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells
Published Date
Feb 2, 2014
Journal
Volume
11
Issue
3
Pages
319 - 324
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