Real-time precise 3D measurement of micro transparent objects using All-In-Focus imaging system

Recently micromanipulation has gained much interest for industrial and biological applications. To realize efficient manipulation of these applications, it is crucial to know the position of microobjects in 3D space. This means that both the micromanipulator and the target object should be detected and located in 3D space in a robust way. A number of different methods for 3D microobject positioning were proposed in the literature; however,...